FIG. 2.
RAD51-K133R and RAD51-K133A expression reduces HDR but increases SSA. Wild-type (wt) ES cells with either the DR-GFP or SA-GFP reporter were cotransfected with the I-SceI and RAD51 expression vectors, as indicated. The efficiency of homologous repair is indicated relative to transfection with the I-SceI expression vector alone, which is set to 1. The asterisks indicate a statistically significant difference from transfection with the I-SceI expression vector alone, with P ≤ 0.0001, except for SA-GFP repair with RAD51-WT, with P = 0.02. The error bars indicate standard deviations.