Table 2.
Primer and probe sequences of target strains and internal standard.
| Primers and probes | Sequence (5′ → 3′) | Amplicon length (base pairs) |
|---|---|---|
| Pf153_F | CGACCATCTTCAAGCCCTTGG | 127 |
| Pf153_R | GACGTTGGGACGGGTATTTCG | |
| Pf153_P | FAM-CCTCATGGCTACGTGGACCAATCACCTT-BHQ1a | |
| DSMZ13134_F | CTCTCCAGCCACTCGTTCAAC | 174 |
| DSMZ13134_R | GCTCAAGTGCTCCACCACC | |
| DSMZ13134_P | FAM-CGAAGAGCCGCCGCCCTACGTCAA-BHQ1a | |
| ACMV_F | CCACAGACAAGATCCACTCTCC | 86 |
| ACMV_R | CACTCTACTCAGGTTCCAATCAAAG | |
| ACMV_P | ROX-ACAGACAATTCAAGAAGCGAGCCATCCG-BHQ2a |
a
BHQ, Black Hole quencher.