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Inactivated Francisella tularensis (iFt)-Mueller Hinton Broth (MHB) elicits greater pro-inflammatory cytokine production compared to iFt-Brain-Heart Infusion (BHI). Bone marrow-derived dendritic cells were incubated with phosphate-buffered saline, iFt-BHI, or iFt-MHB for 24 h. Supernatants were analyzed for (A) IL1B, (B) IL10, (C) IL6, and (D) TNF-α. Values presented are mean ± SE of two independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001.
