Figure 3.

Inactivated Francisella tularensis (iFt)-Mueller Hinton Broth (MHB) better reduces bacterial burden following Ft LVS infection as compared to that of iFt-BHI: mice were immunized as in Figure 2 with phosphate-buffered saline (white bars), iFt-MHB (gray bars), or iFt-BHI (black bars) and challenged with Ft LVS grown in MHB. Subsequently, Ft LVS colony-forming units were enumerated in the lung (A) and spleen (B) homogenates at indicated days postinfection. Each bar represents the mean ± SE (error bar) of two independent experiments with a total of eight mice per group. ***p < 0.001.