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. 2017 Jan 10;7:677. doi: 10.3389/fimmu.2016.00677

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Copyright © 2017 Kumar, Sunagar, Pham, Franz, Rosa, Hazlett and Gosselin.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Inactivated Francisella tularensis (iFt)-Mueller Hinton Broth (MHB) induces superior resistance to Ft LVS-induced systemic tissue damage compared to that of iFt-Brain-Heart Infusion (BHI): mice were immunized on days 0 and 21 with phosphate-buffered saline (white bars), iFt-MHB (gray bars), or iFt-BHI (black bars) and challenged with Ft LVS grown in MHB. Lactate dehydrogenase activity was quantified in serum drawn on indicated days postchallenge. Prechallenge sera were drawn 11 days after boost. Each bar represents mean ± SE (error bar) of two independent experiments with a total of eight mice per group. *p < 0.05.