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. 2017 Jan 10;7:677. doi: 10.3389/fimmu.2016.00677

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Copyright © 2017 Kumar, Sunagar, Pham, Franz, Rosa, Hazlett and Gosselin.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Enhanced binding of inactivated Francisella tularensis (iFt)-Mueller Hinton Broth (MHB) to antigen-presenting cells (APCs) as compared to iFt-Brain-Heart Infusion (BHI). Bone marrow-derived dendritic cells (BMDCs) were incubated with GFP-expressing iFt-BHI or iFt-MHB. Cells were washed and stained with CD11c antibody before analyzing with flow cytometry for GFP signal associated with BMDCs (A). (B) Percentage of GFP-positive (+) BMDCs as evaluated by flow cytometry. Values represent mean ± SE of two independent experiments. ***p < 0.001.