FIG. 1.

Transcriptional activators act on specific regions of the STA1 promoter. (A) Effects of transcriptional activators on STA1 expression. The wild type (WT) and mutants were grown in synthetic medium containing 2% glycerol-ethanol as a carbon source. Glucoamylase activities are averages of the results from three independent experiments. (B) Effects of transcriptional activators on lacZ expression mediated by UAS1-2 and UAS2-1. pLG-UAS1-2 and pLG-UAS2-1 were transformed into the wild type and each mutant, and three independent transformants of each were tested for β-galactosidase activity under derepressing conditions (2% glycerol-ethanol). (C) The STA1 promoter is composed of two UASs, UAS1-2 and UAS2-1. The FRE for Ste12 and Tec1 binding in the STA1 promoter is indicated. UAS1-1 and UAS2-2, to which two major repressors, NRG1 and SFL1, for glucose repression of STA1 expression bind, respectively, are also represented (20).