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. 2004 Nov;24(21):9542–9556. doi: 10.1128/MCB.24.21.9542-9556.2004

FIG. 5.

FIG. 5.

Flo8-Mss11 binding to the endogenous STA1 promoter requires Ste12 and Tec1. (A) The ste12Δ and tec1Δ mutations eliminate Flo8 and Mss11 binding to UAS1-2. FLO8-HA wild-type (WT), FLO8-HA mss11Δ, FLO8-HA ste12Δ, FLO8-HA tec1Δ, MSS11-HA wild-type, MSS11-HA flo8Δ, MSS11-HA ste12Δ, MSS11-HA tec1Δ, and untagged strains were grown in synthetic medium containing 2% glycerol-ethanol to mid-log phase, and anti-HA ChIP assays were performed with 1 mg of total extracts. (B) Ste12 and Tec1 binding to UAS2-1 is independent of Flo8 and Mss11. STE12-HA wild-type, STE12-HA flo8Δ, STE12-HA mss11Δ, TEC1-HA wild-type, TEC1-HA flo8Δ, TEC1-HA mss11Δ, and untagged strains were grown in synthetic medium containing 2% glycerol-ethanol to mid-log phase, and anti-HA (α-HA) ChIP assays were performed as described above with 500 μg of total extracts to determine Ste12 and Tec1 binding to UAS2-1.