TABLE 2.
Plasmids used in this study
| Plasmid | Description | Reference |
|---|---|---|
| pGEX-NRG1 | NRG1 ORFa in pGEX4T-1 | 35 |
| pGEX-FLO8 | Replacement of NRG1 with FLO8 ORF of pGEX-NRG1 | This study |
| pGXE-MSS11 | Replacement of NRG1 with MSS11 ORF of pGEX-NRG1 | This study |
| pRS305 | LEU2 | 40 |
| pRS305-HA | Triple HA tag in pRS305(SalI/XhoI) | This study |
| pRS305-SNF6-HA | SNF6 in pRS305-HA | This study |
| pRS305-RPB3-HA | RPB3 in pRS305-HA | This study |
| pRS325 | 2μm LEU2 | 6 |
| pRS325-HA | Triple HA tag in pRS325(SalI/XhoI) | This study |
| pRS325-ADH1p-HA | ADH1 promoter (−1500 to −1) in pRS325-HA(SacI/NotI) | 20 |
| pRS325-MSS11-HA | MSS11 in pRS325-ADH1p-HA | This study |
| pRS325-STE12-HA | STE12 in pRS325-ADH1p-HA | This study |
| pRS325-TEC1-HA | TEC1 in pRS325-ADH1p-HA | This study |
| pRS326 | 2μm URA3 | 6 |
| pRS326-HA | Triple HA tag in pRS326(SalI/XhoI) | This study |
| pRS326-FLO8-HA | 3.5-kb fragment containing FLO8 ORF in pRS326-HA | This study |
| pRS326-MSS11-HA | 3.7-kb fragment containing MSS11 ORF in pRS326-HA | This study |
| pLG 670-Z | 2μm UAS3 CYC1-lacZ | 15 |
| pLG-STA1-1 | −2105 to −1 region of the STA1 promoter in pLG-670z | 20 |
| pLG-STA1-1a | Deletion of SpeI fragment in pLG-STA1-1 | This study |
| pLG-UAS1-2 | −1905 to −1642 region of the STA1 promoter in pLG-670Z | 20 |
| pLG-UAS2-1 | −1380 to −1147 region of the STA1 promoter in pLG-670Z | 20 |
| pLG-FLO11(UAS1-2) | −1993 to −1730 region of the FLO11 promoter in pLG-670Z | This study |
| pLG-FLO11-1(UAS1-2) | Substitution of CCAAA with GCAAA in pLG-FLO11(UAS1-2) | This study |
| pLG-FLO11b | −1435 to −1202 region of the FLO11 promoter in pLG-670Z | This study |
| pRS425-NRG1-HA | 2.0-kb fragment containing NRG1 ORF in pRS425-HA | 20 |
| pRS425-SFL1-HA | 3.5-kb fragment containing SFL1 ORF in pRS425-HA | 20 |
| pLexA-MIG1 | MIG1 in pSH2-1 | 42 |
| pLexA-FLO8 | FLO8 in pLexA-Mig1 (BamHI/XhoI) | This study |
| pLexA-MSS11 | MSS11 in pLexA-Mig1 (BamHI/SalI) | This study |
| pLexA-STE12 | STE12 in pLexA-Mig1 (BamHI/XhoI) | This study |
| pLexA-TEC1 | TEC1 in pLexA-Mig1 (BamHI/XhoI) | This study |
| pSH18-34 | 2μm URA3 8lexAop-GAL1TATA-lacZ | 10, 46 |
a
ORF, open reading frame.