Table 1.
Dynamic multiple reaction monitoring (DMRM) parameters for phytohormone standards in ammonium acetate (NH4Ac) mobile phase.
| PH | Q1 | Q3 | CE | RT | SM | ISTD | Q1 | Q3 | CE | RT | SM |
|---|---|---|---|---|---|---|---|---|---|---|---|
| ABA | 263.1 | 153.0 | 8 | 3.3 | – | d6-ABA | 269.2 | 159.2 | 8 | 3.3 | – |
| CA | 147.0 | 103.1 | 8 | 2.5 | – | d7-CA | 154.1 | 110.0 | 8 | 2.5 | – |
| GA3 | 345.1 | 143.0 | 32 | 2.8 | – | d2-GA3 | 347.1 | 143.0 | 32 | 2.8 | – |
| GA4 | 331.2 | 243.1 | 16 | 4.5 | – | d2-GA4 | 333.2 | 245.1 | 20 | 4.5 | – |
| IAA | 176.1 | 129.9 | 12 | 1.7 | + | d2-IAA | 178.1 | 132.0 | 12 | 1.7 | + |
| ICA | 160.0 | 115.6 | 12 | 1.8 | – | d2-IAA | 178.1 | 132.0 | 12 | 1.7 | + |
| JA | 209.1 | 59.0 | 8 | 3.5 | – | H2JA | 211.1 | 59.0 | 12 | 4.1 | – |
| OPDA | 291.2 | 165.1 | 16 | 6.7 | – | H2JA | 211.1 | 59.0 | 12 | 4.1 | – |
| SA | 137.0 | 92.9 | 16 | 1.6 | – | d6-SA | 141.0 | 96.9 | 16 | 1.5 | – |
| Zeatin | 220.1 | 135.7 | 20 | 2.9 | + | d5-Zeatin | 225.2 | 137.1 | 20 | 2.9 | + |
PH, phytohormone; Q1, precursor ion selected in Q1; Q3, product ion selected in Q3; CE, collision energy; RT, retention time; SM, scan mode; ISTD, internal standard; ABA, 2-cis-4-trans-abscisic acid; CA, trans-cinnamic acid; GA3, gibberellin A3; GA4, gibberellin A4; IAA, 3-indoleacetic acid; ICA, indole-3-carboxylic acid; JA, jasmonic acid; JA-ile, jasmonoyl-isoleucine; OPDA, 12-oxo phytodienoic acid; SA, salicylic acid. d2-GA3, 17,17-d2-gibberellic acid; d2-GA4, 17,17-d2-gibberellin A4; d2-IAA, indole-3-acetic-2,2-d2 acid; d5-zeatin, d5-trans-zeatin; d6-ABA, d6-2-cis-4-trans-abscisic acid; d6-SA, salicylic-d6 acid; d7-CA, trans-cinnamic-d7 acid; H2JA, dihydrojasmonic acid.