Table 2.

List of primers used in this study.

Primer	Nucleotide sequence 5′–3′	Description
tmRNA-F	TTCTGTGTCAGGGTAAGTTCC	Up PCR fragment for ΔssrA construction
tmRNAKmUp-R	TTATCCATTAAAAATCAAACGGATCACATACCTAAGATGAAGCTATCT
tmRNA-R	ACTGAATCACCTCCTGTTATCG	Down PCR fragment for ΔssrA construction
tmRNAKmDown-F	TACGAGGAATTTGTATCGATGTGGACGTGGGTTCGACT
KmN-F	CCGTTTGATTTTTAATGGATAA	Kmr cassette for ΔssrA construction
KmN-R	CATCGATACAAATTCCTCGTA
pLSROM-F-Xba2	CGTCTGCAAAATACTCTAGAGATGGATCAAG	For pROM construction
pLSROM-R-Xba2	CTCACGAGACAGTCTAGAAAGTACAAAACCTCC
tmRNACj-F	CGCGGATCCTTCATCTTAGGTATGTGATTTC	For ssrA cloning
tmRNACj-R	GCGCAAGCTTGGTCTGTTTGTGACTCCC
tmRNA-1	AATTATCCTGCGCTCCAGAA	For ΔssrA construction and DNA sequencing
tmRNA-2	TTTCAAGACACGGCTGACA

Underlined sequence indicates enzyme restriction sites.