Table 1. Gradient used for fractionation of trypsin-digested peptides by SCX chromatography.
The flow rate is constant at 2.5 mL/min.
| Time (min) | Duration (min) | Gradient (% B) |
|---|---|---|
| 0 | N/A | 0 |
| 5 | 5 | 0 |
| 10 | 5 | 10 |
| 46 | 36 | 40 |
| 50 | 4 | 100 |
| 55 | 5 | 100 |
| 56 | 1 | 0 |
| 60 | 4 | 0 |
The flow rate is constant at 2.5 mL/min.
| Time (min) | Duration (min) | Gradient (% B) |
|---|---|---|
| 0 | N/A | 0 |
| 5 | 5 | 0 |
| 10 | 5 | 10 |
| 46 | 36 | 40 |
| 50 | 4 | 100 |
| 55 | 5 | 100 |
| 56 | 1 | 0 |
| 60 | 4 | 0 |