Table 2. Gradient setting on Easy nLC 1000 instrument for nano-LC-MS/MS analysis of peptides.
The flow rate is constant at 300 nL/min.
| Time (min) | Duration (min) | Gradient (% B) |
|---|---|---|
| 0 | N/A | 5 |
| 160 | 160 | 30 |
| 170 | 10 | 95 |
| 180 | 10 | 95 |
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