Table 3.

Mass spectrometry parameter settings on Q Exactive for acquiring LC-MS/MS data.

MS parameter	Value
Full MS
Microscans	1
Resolution (at m/z 200)	70,000
Automatic gain control target	1.00E+06
Maximum ion time	50 ms
Scan range	350–1600
dd-MS2
Microscans	1
Resolution (at m/z 200)	17,500
Automatic gain control target	1.00E+05
Maximum ion time	250 ms
Loop count	12
Isolation window	3 m/z
Fixed first mass	100 m/z
Normalized collision energy	24
dd settings
Underfill ratio	5%
Intensity threshold	1.00E+04
Charge exclusion	Unassigned, 1, ≥5
Peptide match	on
Exclude isotopes	on
Dynamic exclusion	20 s