Skip to main content
. Author manuscript; available in PMC: 2017 Jan 10.
Published in final edited form as: Curr Protoc Protein Sci. 2016 Nov 1;86:24.8.1–24.8.19. doi: 10.1002/cpps.16

Table 5. Laser-puller P-2000 settings.

These parameters were used to pull 10-μm tips from 360-μm o.d. × 75 μm i.d. fused silica tubing on our laser puller. The parameters on individual laser puller should be optimized following the instruction of the vendor.

Line HEAT FIL (Filament) VEL (Velocity) DEL (Delay) PULL
1 300 0 30 128 0
2 250 0 20 128 0
3 175 0 12 128 0
4 155 0 9 128 0