Table 1.
Treatment | Description | |
---|---|---|
(1) | SW+BI_4 | SW inoculated with BI (Control), 4°C |
(2) | SW+BI_24 | SW inoculated with BI (Control), 24°C |
(3) | SW+SI+oil_4 | SW inoculated with SI+LLS oil, 4°C |
(4) | SW+SI+oil_24 | SW inoculated with SI+LLS oil, 24°C |
(5) | SW+BI+oil_4 | SW inoculated with BI+LLS oil at 4°C |
(6) | SW+BI+oil_24 | SW inoculated with BI+LLS oil, 24°C |
(7) | BW+SI _4 | BW inoculated with SI (Control), 4°C |
(8) | BW+SI _24 | BW inoculated with SI (Control), 24°C |
(9) | BW+SI+oil_4 | BW inoculated with SI+LLS oil, 4°C |
(10) | BW+SI+oil_24 | BW inoculated with SI+LLS oil, 24°C |
(11) | BW+BI+oil_4 | BW inoculated with BI+LLS oil, 4°C |
(12) | BW+BI+oil_24 | BW inoculated with BI+LLS oil, 24°C |
Light Louisiana sweet crude oil (LLS) was directly added at a final concentration of 200 ppm. SW- filtered surface water; SI- unfiltered surface water (inoculum); BW-filtered bottom water; BI- unfiltered bottom water (inoculum). 20 ml of filtered water and 2.5 mL of unfiltered water/inoculum were added. Water samples were filtered through 0.20 μm polycarbonate filters. Incubation was conducted at 4 or 24°C in the dark.