Table 2.
Comparison of parameters of 1D and 2D NMR techniques
| Parameter | 1D | 2D |
|---|---|---|
| Degree of overlap for quantitative analysis | Higher | Lower |
| Sensitivity to pulse imperfections | Lower (lower signal to noise ratio) | Higher |
| Number of factors influencing peak | Lower | Higher (e.g., J-coupling or relaxation time) |
| Calibration procedure | One for the whole experiment | Individual for each compound (for quantitative analysis—peaks ratio does not reflect the concentration ratio directly) |
| Experiment duration | Shorter | Longer |
| Efficacy (particularly for small molecules) | Lower | Higher |