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. 2016 Dec 15;469(2):213–223. doi: 10.1007/s00424-016-1916-4

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© The Author(s) 2016

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 7 — Effect of Kv7 channel openers in PIP₂- and Gβγ-depleted cells. Mean data showing action of Retigabine, S-1 and NS15370 in HEK293 Kv7.4 cells under various conditions (control, preincubated with wortmannin alone, preincubated with wortmannin + short (≤30 s) application of trypsin, preincubated with gallein). Currents after treatment with pan-Kv7 channel blocker linopirdine are shown for reference. *, **, *** and **** denote p < 0.05 – p < 0.0001, respectively, compared to controls