Fig. 3.

Splicing of wild type and mutant inteins in model precursors. MSP (Mle DnaB intein) and MLP (Msm DnaB-1 intein) were expressed at 15 °C and MVP (Bvi IcmO intein) was expressed at 30 °C. In vivo splicing of soluble proteins was examined after electrophoresis in SDS-PAGE followed by staining with Simply Blue Safe Stain. Residues mutated to alanine are listed above each lane. Lane S contains the NEB 10–250 kDa Protein Ladder with molecular masses (kDa) listed on the left. MIP precursor with the indicated inteins, MP spliced product, I excised intein, WT wild type intein