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Determination of detection limits of RT-PCR assays targeting the 23S rRNA (A) and groEL mRNA (B). LightCycler RT-PCRs were performed with the two-enzyme system. A pooled platelet concentrate was spiked with a fresh overnight culture of E. coli ATCC 35218 (109 cells) and diluted with platelet concentrate without bacterial contamination down to 1 CFU per ml. Sterile H2O was used as a negative control.
