FIG. 3.
Time course of 23S rRNA and groEL mRNA stabilities in ethanol-treated E. coli cells. Gel electrophoresis of RT-PCR products of the 23S rRNA (A) and the groEL mRNA (B) of E. coli ATCC 35218 was performed after LightCycler RT-PCRs as described in Materials and Methods. E. coli cells (109 cells per ml, lane 9) were inactivated by incubation with 67% ethanol for 7 min at room temperature for 5 (lane 4), 15 (lane 5), 45 (lane 6), and 70 min (lane 7). Lane 3 contains the untreated 1-to-100 dilution of E. coli, lane 8 contains the no-template control, and lane 1 contains the DNA molecular weight marker.