TABLE 2.
Oligonucleotide primers and TaqMan fluorescent probe sequences used for RT-PCRs
| Oligonucleotide | Target gene | Sequence (5′ to 3′) | Position of product (nt) | GenBank accession no. | Reference or source |
|---|---|---|---|---|---|
| BLF1380 | 23S rRNA | GGACAACAGGTTAATATTCC | 1380-1398 | AF053966 | 36 |
| BLR1686 | 23S rRNA | GGGGCCATTTTGCCGAGTTC | 1685-1666 | AF053966 | 36 |
| BLF-E | 23S rRNA | TTCCTGTACTTGGTGTTACTGCGA | 1395-1418 | AF053966 | This work |
| BLR1686-E | 23S rRNA | GGCACCATTTTGCCTAGTTC | 1685-1666 | AF053966 | This work |
| BL-1-TM | 23S rRNA | [6-FAM]ACCTGTGTCGGTTTGSGGTACGRTT[TAMRA] | 1620-1596 | AF053966 | This work |
| H279 | groEL | GAIIIIGCIGGIGAYGGIACIACIAC | 247-272 | X82212.2 | 12 |
| H280 | groEL | YKIYKITCICCRAAICCIGGIGCYTT | 855-830 | X82212.2 | 12 |
| HSP60-73F | groEL | TGAAACGYGGTATCGACAAA | 347-366 | X82212.2 | This work |
| HSP60-287R | groEL | CTGCATACCTTCAACMACGTCC | 583-562 | X82212.2 | This work |
| HSP60-217TM1 | groEL | [6-FAM]CCTTCTTTACCGACTTTITCCATCGCTT[TAMRA] | 519-492 | X82212.2 | This work |
| HSP60-217TM2 | groEL | [6-FAM]CCTTCTTTRCCMACTTTMTCCATCGCTT[TAMRA] | 519-492 | X82212.2 | This work |