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. 2016 Nov;186(11):3040–3053. doi: 10.1016/j.ajpath.2016.07.019

Figure 5.

Figure 5

High neuropeptide Y (NPY) and Y5 receptor (Y5R) immunoreactivity marks invasive neuroblastoma (NB) cells in TH-MYCN mice. A and B: Representative magnetic resonance image (MRI) of a primary tumor (T) and liver metastasis (M) in the TH-MYCN mouse model of NB. C and D: Hematoxylin and eosin (H&E) staining of the primary tumor and liver metastases derived from TH-MYCN mice. E: NPY immunostaining in TH-MYCN tumors. NPY-positive cells within the primary tumor (red arrows) accumulate around blood vessels (V) and in the blood stream. F: Representative image of NPY immunostaining in liver metastasis derived from a TH-MYCN mouse. All cells within the lesion are highly positive for NPY. G–I: Y5R immunostaining in primary tumors arising in TH-MYCN mice. Groups of highly Y5R-positive NB cells (red arrows) are detectable around the vessels (G), penetrating the vessel wall (H) and in the vessel lumen (I). J: Representative image of Y5R immunostaining in liver metastasis derived from a TH-MYCN mouse. All cells within the lesion are positive for Y5R. The images are shown at various magnification levels to highlight the patterns of immunostaining seen within tumor tissue. Scale bar = 50 μm (C–J).