Figure 11.

Representative immunofluorescence confocal photomicrographs demonstrating gliosis and localizing sites of SQSTM1 immunoreactivity within the glomerular region of the olfactory bulb in diacetyl-exposed mouse. A: SQSTM1 (red) puncta are within the cytoplasm of allograft inflammatory factor 1 (AIF1)–positive (white) reactive microglial cells (arrows) in a mouse inhaling 200 ppm diacetyl. B: In the merged image of OMP and AIF1 immunostaining, the AIF1-positive (white) microglial cells accumulating the SQSTM1 (red) and the scattered SQSTM1 particles are near neurons expressing OMP (green) in a mouse inhaling 200 ppm diacetyl. C: High-magnification image of an AIF1-positive (white) reactive microglial cell containing intracytoplasmic SQSTM1 (red) puncta in a mouse inhaling 200 ppm diacetyl. D: In the high-magnification merged image of OMP and AIF1 immunostaining, OMP (green) puncta are also present in AIF1-positive cells of the diacetyl-exposed mouse. Scale bars: 10 μm (A and B); 5 μm (C and D).