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. 2016 Nov;186(11):2887–2908. doi: 10.1016/j.ajpath.2016.07.018

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© 2016 American Society for Investigative Pathology. Published by Elsevier Inc. All rights reserved.

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Low-magnification immunofluorescence localizing sites of SQSTM1 immunoreactivity within the olfactory bulb of the diacetyl-exposed mouse. A: Photomicrograph of immunofluorescence staining of SQSTM1 in red within the glomerular region of the mouse olfactory bulb, showing scattered submicrometer-sized immunoreactive SQSTM1 and larger accumulations (arrows) in specific foci. B: Photomicrograph of the same region shown in A, showing SQSTM1 in red, OMP in green, and DAPI in blue. The scattered SQSTM1 immunoreactivity localizes within or near OMP-positive axons. The clusters of SQSTM1-positive particles (arrows) appear to be within cells near the OMP-positive axons. Scale bar = 50 μm (A and B).