Table 3.
Primary Antibodies Used for Immunofluorescence Assays
| Antigen | Host/clone | Company | Catalog no. | Dilution |
|---|---|---|---|---|
| Catenin β-1 (CTNNB1) | Rabbit/E247 | Abcam (Cambridge, MA) | ab32572 | 1:100 |
| Keratin, type II cytoskeletal 8 (KRT8)∗ | Rabbit/EP1628Y | Abcam (Cambridge, MA) | ab53280 | 1:200 |
| Cadherin-1 (CDH1) | Mouse/36 | BD Biosciences (San Jose, CA) | 610181 | 1:50 |
| Olfactory marker protein (OMP) | Goat (polyclonal) | Wako (Richmond, VA) | 544-10001 | 1:100 |
| Ubiquitin, conjugated and unconjugated | Mouse/Ubi-1 | Millipore (Billerica, MA) | MAB1510 | 1:400 |
| Ubiquitin (linkage-specific, K63) | Rabbit/EPR8590-448 | Abcam (Cambridge, MA) | ab179434 | 1:100 |
| Allograft inflammatory factor 1 (AIF1) | Chicken (polyclonal) | Abcam (Cambridge, MA) | Ab139590 | 1:200 |
| Mouse lysosomal membrane glycoprotein 1 (LAMP1) | Rat/monoclonal† | Developmental Studies Hybridoma Bank (Iowa City, IA) | 1D4B | 1:50 |
| Mouse lysosomal membrane glycoprotein 2 (LAMP2) | Rat/monoclonal† | Developmental Studies Hybridoma Bank (Iowa City, IA) | ABL-93 | 1:50 |
| SQSTM 1/p62 | Rabbit (polyclonal) | Lifespan Biosciences, Inc. (Seattle, WA) | LS-B4617 | 1:200 |
∗
This antibody also recognizes keratin, type I cytoskeletal 18 (KRT18). Both KRT8 and KRT18 are cytoskeletal components of airway epithelial cells.45
†
The monoclonal antibody developed by J. Thomas August was obtained from the Developmental Studies Hybridoma Bank, generated by the National Institute of Child Health and Human Development of the NIH, and maintained at the Department of Biology, The University of Iowa (Iowa City).