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. 2016 Nov;186(11):2813–2823. doi: 10.1016/j.ajpath.2016.07.021

Figure 1.

Figure 1

DR3 expression and sources of TL1A in the peritoneal cavity. Inflammation in the cavity was induced via an i.p. injection of Staphylococcus epidermidis supernatant (SES). Peritoneal membranes were harvested, fixed, cut into sections, and stained for DR3 expression or analyzed for the relative quantity (RQ) of TL1A mRNA by quantitative RT-PCR. A: Representative images showing DR3 staining in the mesothelial layer of DR3+/+ mice after 0 and 12 hours but absent after 24 hours of inflammation. B: Summary of DR3 expression in the mesothelial layer. The % positive (brown) pixels within the mesothelial layer of the membrane is shown. C: RQ of TL1A mRNA in the peritoneal membrane of DR3+/+ and DR3−/− mice. Not significantly different by analysis of variance. D: RQ of TL1A mRNA in DR3+/+ and DR3−/− resident peritoneal macrophages 1 hour after stimulation using SES. Macrophages were sorted from other leukocytes found in the peritoneal cavity from 17 pooled DR3+/+ and DR3−/− mice, challenged with SES, and analyzed for RQ of TL1A mRNA. Data shown from four replicate experiments. Data are shown as means ± SEM (B–D). n = 3 to 6 (B, DR3+/+ mice and DR3−/− mice per time point); n = 5 (C, mice per time point); n = 4 (DR3+/+, naive and SES; DR3−/− SES) or 5 (DR3−/− naive) (D). ∗P < 0.05 by t-test assuming unequal variance, ∗∗∗P < 0.001 by one-way analysis of variance. Scale bar = 25 μm (A).