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. 2016 Nov;186(11):2813–2823. doi: 10.1016/j.ajpath.2016.07.021

Figure 3.

Figure 3

Chemokine production in the peritoneal cavity of DR3+/+ and DR3−/− mice after Staphylococcus epidermidis supernatant (SES)–induced inflammation. Inflammation in the cavity was induced via an i.p. injection of SES. Levels of the indicated chemokines were measured in the following: cell-free supernatants in peritoneal lavage by enzyme-linked immunosorbent assay (A) and mRNA levels in the peritoneal membrane by quantitative RT-PCR (B). Representative data from one of two experiments. Overall male/female ratio was 50:50 and matched for DR3+/+ and DR3−/− groups. C: Production of chemokine ligand (CCL) 3 and CCL4 by resident macrophages derived from DR3+/+ and DR3−/− mice, stimulated with SES. Each point represents a culture. Values lower than the limit of the assay were assigned the lowest limit for generation of means and statistical testing. Dotted line shows lowest limit of assay. Statistical analysis by analysis of variance and Bonferroni post hoc test showed significant differences between DR3+/+ and DR3−/− mice. Data represent means ± SEM (A–C). n = up to 6 (A and B, DR3+/+ and DR3−/− mice per time point). ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001. BCA, B-cell attracting chemokine; IP, IFN-γ-inducible protein; KC, keratinocyte chemoattractant; LIX, LPS-induced CXC chemokine; MCP, monocyte chemoattractant protein; MIP, macrophage inflammatory protein; RANTES, regulated on activation normal T cell expressed and secreted.