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Collagen (Col) deposition increases in the kidney from A2AR−/− mice. Collagen I and collagen III expression (arrows) increase in the interstitium (A–C), and collagen IV deposition (arrows) enhances in the glomeruli from nephritic kidneys (A and D) in A2AR−/− mice. Collagen staining was used. Results are expressed as means ± SEM (B–D). ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001. Scale bar = 200 μm (A). Original magnification, ×200 (A). GN, glomerulonephritis; KO, knockout; Nl, normal; WT, wild type.
