Figure 8.
Lack of macrophage A2AR increases kidney injury in nephrotoxic serum (NTS) nephritis. A: Periodic acid-Schiff staining of kidney sections of nephritic kidneys from mice that were depleted (Depl) of macrophages (Mφ) and received: adoptive transfer of wild-type (WT) macrophages (Depl AT WT Mφ, AT WT Mac); adoptive transfer of WT Mφ and treated with A2AR agonist (Depl AT WT Mφ + A2AR agonist, AT WT Mac Tx); adoptive transfer of Mφ deficient in A2AR and treated with A2AR agonist (Depl AT KO Mφ + A2AR agonist, AT KO Mac Tx); and adoptive transfer of A2AR−/− macrophages without treatment with A2AR agonist (Depl AT KO Mφ, AT KO Mac). Sections were sampled on day 8 after NTS injection. B: Quantitation of kidney injury. Data are expressed as means ± SEM (B). ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗∗P < 0.0001. Scale bar = 60 μm (A). Original magnification, ×400 (A). Glom, glomerular.