Figure 1.

Neddylation inhibition in T cells. Analysis by Western blot of Cullin-1 protein in CD90.2⁺ splenic T cells. Loss of higher-molecular-weight band of Cullin-1 by treatment with 100 nmol/L (A) and 500 nmol/L (B) MLN4924 indicates level of inhibition of nedd8 attachment to cullin protein. α-Tubulin protein levels were analyzed as an equal loading control. C: CD90.2⁺ T-cell viability by annexin V staining after incubation with MLN4924 at indicated concentrations for 72 hours. One representative experiment each of three is shown. ^∗∗∗P < 0.001 versus 0 h.