Figure 5.

Effect of Rg3 on COX-2 protein inhibition in human asthmatic airway epithelial tissues. (a) Human asthmatic lung tissue was treated with Dex for positive control or Rg3, and whole tissue lysates were prepared and then subjected to western blot analysis with anti-COX-2 antibodies. Anti-β-actin antibody was used as an internal control. The blots were normalized by β-actin expression levels and compared to the saline-treated tissue. (b) Normalized COX-2 expression was measured by Image J program and represented as bar charts. Statistical significance was determined by unpaired t test (one-tail) compared to Con (only PBS-treated asthmatic lung tissue). Values are means ± SEM; ^∗P< 0.05; ^∗∗P< 0.01.