Table 3.

Oligonucleotide sequences of primers used in this study

Primer name	Nucleotide sequence (5′→3′)	Position in 45S region
45SF	GCGAGAATTCCACTGAACC	[12]
45SR	ACGAATTCCCTCCGCTTATTG ATATGCTTA	[12]
KgF	GACCACCCTTGGGTTGA (G→T)	170–186
G-1F	TCTAAAACACAAACGACTCTAGG (C→A)	283–305
AgF	TCACTCCTTTGCGGGAATC	477–495

Note. Bold underlined nucleotide is the additional mismatch introduced via substitution of G for T and C for A.