Fig. 3.

Gain of integrin-α8 expression mediates MM cell invasion and migration. (A) Integrin-α8 overexpressed cells that invaded through a basement membrane coated 8 μM pore transwell were used for this invasion assay. Integrin-α8 overexpressed LP1 and RPMI 8226 cells were starved for 24 h, after which cells that invaded to the bottom were counted. (B) An 8 μM pore transwell migration assay was used. Integrin-α8 overexpressed MM cells were starved for 24 h, and then allowed to migrate to the bottom well RPMI/IMDM serum free media supplemented with SDF-1a (0 or 30 nM). (C) The CXCR4 mRNA and protein level in integrin-α8 overexpressed MM cells were confirmed by qRT-PCR and Western blot, respectively. The mRNA data shown are the mean fold changes ± SD of three independent experiments compared to the control. Gene expression values in the samples were divided by those in the controls (i.e. each gene in the control is ‘1’). (D) Adhesion of overexpressed integrin-α8 LP1 and RPMI 8266 cells to FN or MM patient isolated BMSCs coated 96 well microplates. Unattached cells were washed out, after which adherent cells were determined based on the O.D. value.