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. 2017 Jan 10;7:37049. doi: 10.1038/srep37049

Figure 3. Knockdown of YAP or treatment with dobutamine prevents expression of mRNAs of AREG and TEAD1 induced by loss of LSR.

Figure 3

(a) Western blotting for LSR and pYAP in LSR-knockdown Sawano cells with or without siRNA-YAP and 10 μM dobutamine. (b) Immunocytochemical staining for LSR and pYAP in LSR-knockdown Sawano cells with and without siRNA-YAP or 10 μM dobutamine. Scale bars: 20 μm. (c–f) Real-time PCR for mRNAs of AREG and TEAD1 in LSR-knockdown Sawano cells with and without siRNA-YAP or 10 μM dobutamine. The results are shown as bar graphs. Control vs. siRNA-LSR: **p < 0.01. siRNA-LSR vs. siRNA-LSR + YAP and siRNA-LSR + dobutamine: ##p < 0.01.