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. 2004 Oct;42(10):4840–4842. doi: 10.1128/JCM.42.10.4840-4842.2004

TABLE 1.

Comparison of the PFGE, MLST, flaA, and flaB sequencing results for sporadic and outbreak-associated Campylobacter isolatesi

Species and strain no. Origin Epidemiologic implication PFGE (SmaI)a MLST STb flaA allelec flaB allelec Comment
C. jejuni
    B01751 Human Y S ST 21 245 226 Outbreak 1d
    B01755 Human Y S ST 21 245 226 Outbreak 1
    B01758 Human Y S ST 21 226 226 Outbreak 1
    B01759 Human Y S ST 21 226 226 Outbreak 1
    B01766 Cow feces Y S ST 21 245 226 Outbreak 1
    B01768 Cow feces Y S ST 21 245 226 Outbreak 1
    B01769 Cow feces Y S ST 21 245 226 Outbreak 1
    B01770 Cow feces Y S ST 21 245 226 Outbreak 1
    B01513 Human N T ST 823 117 117 Outbreak 1 (control)
    B02098 Human Y A ST 575 239 177 Outbreak 2e
    B02100 Human Y A ST 575 239 177 Outbreak 2
    B02122 Skim cream N L ST 861 121 103 Outbreak 2 (control)
    B02184 Cow feces N E ST 45 2 307 Outbreak 2 (control)
    *D2678 Human Y 8f ST 21 49 326 Outbreak 3
    *D2692 Raw milk Y 8 ST 21 326 326 Outbreak 3
    Ob5647 Human Y V ST 824 105 260 Sporadic 1
    Ob5815 Human Y V ST 824 105 260 Sporadic 1
    Ob6055 Human Y V ST 824 105 260 Sporadic 1
    Ob6965 Human Y W ST 21 32 103 Sporadic 2
    Ob7119 Human Y W ST 21 32 103 Sporadic 2
    Ob7420 Human Y W ST 21 32 103 Sporadic 2
    EB1410 Human Y M ST 822 5 5 Sporadic 3
    EB1430 Human Y M ST 822 5 5 Sporadic 3
    EB1599 Human Y M ST 822 5 5 Sporadic 3
    A57511 Human Y N ST 658 5 5 Sporadic 4
    A58515 Human Y N ST 658 5 5 Sporadic 4
    SL5512 Human Y O ST 50 36 36 Sporadic 5
    SL5546 Human Y O ST 50 36 36 Sporadic 5
    EB87 Human Y P ST 572 14 96 Sporadic 6
    EB101c Human Y Q ST 572 14 96 Sporadic 6
    EB101j Human Y Q ST 572 14 96 Sporadic 6
    A1689 Human Y R ST 607 14 14 Sporadic 7
    A2891 Human Y R ST 607 14 14 Sporadic 7
    Ob4699 Human Y AA ST 21 121 103 Sporadic 8 (mixed infection)
    Ob5876 Human Y Y ST 50 36 36 Sporadic 8
    Ob6054 Human Y Y ST 50 36 36 Sporadic 8
    Ob6560 Human Y Y ST 50 36 36 Sporadic 8
    Ob6343 Human Y Z ST 22 232 309 Sporadic 9
    Ob6563 Human Y Z ST 22 232 309 Sporadic 9
    Ob4819 Human Y F ST 52 57 57 Sporadic 10
    *Ob5646 Human Y g ST 52 57 57 Sporadic 10
    A21989 Human Y J ST 658 5 5 Sporadic 11 (mixed infection)
    A23530 Human Y I ST 572 105 260 Sporadic 11
    A25293 Human Y I ST 572 105 260 Sporadic 11
    Ob6185 Human Y L ST 842 121 103 Sporadic 12 (mixed infection)
C. coli
    *Ob6057 Human Y G NTh 228 308 Sporadic 12
    *Ob6562 Human Y X NT 229 310 Sporadic 13
    *Ob7415 Human Y X NT 229 310 Sporadic 13
    *Ob7852 Human Y X NT 229 310 Sporadic 13
a

PFGE type names are arbitrary.

b

MLST sequence types were assigned using the MLST website (http://www.mlst.net).

c

fla alleles were assigned using the Campylobacter FlaA Variable Region Database (http://phoenix.medawar.ox.ac.uk/flaA/).

d

Three further outbreak isolates with epidemiological implications (Y) showed the same typing results (MLST was not done).

e

Eighteen further outbreak isolates with epidemiological implications (Y) showed the same typing results (MLST was not done).

f

PFGE types were numbered arbitrarily (relation to the other PFGE patterns is unknown).

g

Did not restrict with SmaI.

h

NT, not typeable.

i

Strains marked by an asterisk were not included for discriminatory index and concordance calculations. Boldface indicates intragenomic recombination events.