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. 2017 Jan 10;7:40487. doi: 10.1038/srep40487

Figure 3. Genetic ablation of osteoclastic miR-214-3p prevents osteolytic bone metastasis in BCX nude mice.

Figure 3

Q-PCR analysis of the miR-214-3p levels (a) and the TRAP and CTSK mRNA levels (b) in osteoclasts and non-osteoclasts isolated from bone marrow cells by MACS. (c) ELISA assay of serum CTX-I levels. (d) The representative images of TRAP staining in distal femur. Scale bar: 100 μm (e) Bone histomorphometric analysis for Oc.S/BS and N.Oc/B.Pm in proximal tibia. (f) The representative bioluminescence imaging showing the degree of metastasis. (g) The number of nude mice with/without bone metastasis in the forelimb, hind limb and spine area detected by bioluminescence imaging. (h) The bioluminescence signal intensity at the sites of bone metastasis (left) and non-bone metastasis (right). (i) The representative micro-CT images showing the osteolytic bone lesion at distal femur. Scale bar: 1 mm (j) The number of osteolytic lesion at distal femur. Note: n = 8 for each group. (a,b) CKO: osteoclast-specific miR-214-3p knockout nude mice, WT: Wild-type nude mice. (c–j) WT: WT nude mice with BCX, CKO: CKO nude mice with BCX. Data were means ± s.d. **P < 0.01.