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. 2017 Jan 10;7:40285. doi: 10.1038/srep40285

Figure 4. Microarray gene expression analysis.

Figure 4

(A) Signal intensity plot for individual chip probes when comparing iCM (cardiac TF and microRNA) to control HDF. (B) Volcano plot displaying the relationship between P-Value determined using ANOVA versus calculated fold change for each probe. Comparison between iCM (cardiac TF and microRNA) and control HDF. Including only probes with defined fold change (< or >1.5) and significant P-Value (<0.05). (C) Fold change and P-Value of selected genes previously described as playing a role in cardiac cell development or function. Fold change is shown for cells transdifferentiated in the presence of cardiac TF and microRNA or cardiac TF only (Red: 413.39, Green: −2.43). (D) Molecular pathways associated with significantly upregulated genes when comparing iCM to control HDF as determined by the WEB-based GEne SeT AnaLysis Toolkit (WebGestalt). The “Gene #” column refers to the number of identified genes that belong to a particular pathway and the “P-value” column refers to the P-value of each of the pathways and based on the number of identified genes. (C) reference gene number in category, O: number of genes in gene set and category, E: expected number in category, R: enrichment ratio. (E) Principal component analysis performed on normalized signal values for each of the chip probes as well as probes from previously published studies.