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. 2017 Jan 10;7:40285. doi: 10.1038/srep40285

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Copyright © 2017, The Author(s)

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(A) Immunofluorescence of transdifferentiated cells (ACTN2/TNNT2) during exposure to small molecule inhibitors: Janus protein tyrosine kinase 1 (JAK1i), Sodium Butyrate (HDACi), SB431542 (TGFβi), CHIR99021 (GSK3i). (B) Number of nuclei, (C) Number of ACTN2⁺ cells, and (D) Number of TNNT2⁺ cells (per mm²). (E) Number of Ki67⁺ nuclei normalized to the total number of nuclei. (F) Number of ACTN2⁺ cells normalized to the total number of nuclei. (G) Number of TNNT2⁺ cells normalized to the total number of nuclei. Experiment performed in triplicate. 4 images were analyzed for each experiment. Error bar represents calculated standard deviation. Significant difference between two values was calculated using t-test (two-tailed distribution, two sample unequal variance).