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. 2017 Jan 10;7:40166. doi: 10.1038/srep40166

Figure 2. Connexin expression and plaque formation in HeLa cells transfected with WT and mutant Cx32.

Figure 2

These are digital images of HeLa cells that have been transiently transfected to express one of the indicated mutations as well as empty vector (vector) and wild type Cx32 (WT). The cells were immuno-stained for Cx32 (red) and counterstained with DAPI (blue) to label nuclei. Some of the PNS + CNS mutants-F51L, T55I, E102G, R142W, R164W, C168Y and V177A - never displayed gap-junction plaques, while some - V139M, R142Q, and R164W – had gap junction plaques (arrows), as did all of the PNS-only mutants (Y151C, V181M, R183C and L239I) and WT Cx32. Scale bar = 10 microns.