Figure 4. Heterotypic pairing of PNS-only mutants with WT Cx32 confirms normal gap junctional properties.

Macroscopic junctional currents for heterotypically paired cells expressing the Y151C (a), V181M (c), R183C (e) and L239I (g) and the corresponding steady state G_j − V_j relations for homotypically paired cells (b,d,f,h, respectively). Mutants show G_j − V_j symmetry similar to that seen in homotypically paired Cx32WT. In (b,d,f,h), data are shown as mean +/− SEM. Solid lines are Boltzmann fits to the data shown by the filled squares. V_j is applied to the cell expressing the second connexin listed in the pairing configuration. See Table 3 for fit parameters.