Figure 5. Heterotypic pairing of plaque-forming PNS + CNS mutants with WT Cx32 confirms the presence of two of the mutants at the plasma membrane.

(a) Representative current traces recorded from an R142Q/Cx32WT pairing; Current responses were increasingly activated when negative pulses were applied to the Cx32 expressing cell. (b) Average normalized G_j − V_j relations for heterotypic R142Q/Cx32WT channels. (c) Representative current traces recorded from a R164W/Cx32WT pairing; Current responses were increasingly activated when negative pulses were applied to the Cx32 expressing cell. (d) Average normalized G_j − V_j relations for heterotypic R164W/Cx32WT channels. In (a) data was filtered at 500 Hz for display. In (c) data was filtered at 200 Hz for display. In (b,d) data are shown as mean +/− SEM. V_j is applied to the cell expressing the second connexin listed in the pairing configuration.