Figure 5.

Co-existence of related recombinant structures and their substoichiometric shifting. (A) Semi-quantitative PCR using structure-specific primers with 28 and 35 cycles was performed to detect the structures and estimate their abundance in leaves of the selected O. rufipogon accessions. The products that could be detected with strong signals by 28 cycles are present in high copy numbers, while those could be detected only by 35 cycles are at low copy numbers. The nuclear gene OsActin1 was used as a control. (B) Estimation of copy numbers per cell of S367, S276, and S352b in leaves by quantitative PCR. Actin1 (two allelic copies per cell) was used as the internal control for normalization.