Table 1.
Details of primers used for RT-qPCR
| Gene | Abbrev. | Sequence | Product length (bp) |
|---|---|---|---|
| Piwi-Like RNA-Mediated Gene Silencing 1 | PIWIL1 | F:CTGGTTCTCTCGCTGTGTGT | 90 |
| R:TTCCAAGCCCTTAGAGCAGC | |||
| Spermatogenesis Associated 4 | SPATA4 | F:CTCTCGATCACCATCCTGCC | 106 |
| R:CTCAATGCACTCATGCTCGC | |||
| Inhibin beta A | INHBA | F:AGGTGGTGGATGCTCGAAAG | 125 |
| R:GGTCTCCTGACACTGCTCAC | |||
| Forkhead box O3 | FOXO3 | F:ATGGCAACCAGACACTCCAG | 97 |
| R:CTGGCCTGAGACATCAAGGG | |||
| Phosphatase and tensin homolog | PTEN | F:CGGCCGTTCCGAGGATT | 99 |
| R:CTGGATGGTTGCAGCGACT | |||
| Cytochrome P450, Family 51, Subfamily A, Polypeptide 1 | CYP51A1 | F:TACCTACCTGCTGGGGAGTG | 107 |
| R:TCCCAAACACAGGTGTCGTC | |||
| Glyceraldehyde-3-phosphate dehydrogenase | GAPDH | F:CTGCTGACGCTCCCATGTTTGT | 150 |
| R:TAAGTCCCTCCACGATGCCAAA |