Fig. 3.

The AGTR1 is a direct target of miR-34a-5p in OS cells. The levels of miR-34a-5p (a and b), the AGTR1 mRNA (c and d) and protein (e) in the miR-34a-5p mimic (5PM) transfected G-292 cells and the miR-34a-5p antagomiR (5PA) transfected SJSA-1 cells versus the negative control (NC), determined by qRT-PCR or Western analyses. f The sequences of the wild-type and mutant 3'-UTR region of AGTR1 gene. The perfectly matched region of AGTR1 3'-UTR with miR-34a-5p were marked in shadow. g The relative luciferase activity (fold) of the reporter with wild-type (WT) AGTR1-UTR or mutant were determined in the miR-34a-5p mimic (in G-292) or antagomiR (in SJSA-1) or Mock transfected OS cells. The reporter without AGTR1-UTR (Vec) was used as a reference. The Renilla luciferase activity of a co-transfected control plasmid was used to control the transfection efficacy. The representative results from three independent experiments shown. *P value < 0.05; **P value < 0.01