Fig. 2.

Sensitivity of the OCCC cell lines TOV-21, OVTOKO and ovarian serous cancer cell line OV-90 towards the PARP inhibitor BMN673 after knockdown of MRE11. Treatment with the PARP inhibitor BMN673 led to moderately decreased cell viability in (a) MRE11-depleted TOV21 (p = 0.0005) but not in (b) MRE11-depleted OVTOKO (p = 0.2) and OV-90 (c) (p = 0.8) compared to the respective controls. P-values indicate the differences in the IC50 values as calculated with an F-test using GraphPad Prism Version 6. The plotted values represent the mean surviving fraction and the error bars represent the standard error of mean (SEM). Western blot analysis (d) showed a decrease but not a loss of MRE11 expression in all the three cell lines after siRNA treatment and all the knockdown experiments were performed two times independently each. All the experiments were performed in triplicate 6-well plates