Table 4.

Mouse histone variants with poor annotation status in the UniProtKB database

Names	Accession number	Protein status	Method of detection			References
			This study (number of identified peptides)	Other MS-based studies	Not MS-based studies
H1.0 (H1°)	P10922	Transcript	Yes (5)	Yes	RT-PCR; WB	[128]
TS H1.7 (H1T2, HANP1)	Q8CJI4	Transcript	Yes (6)		NB; WB	[55]
OO H1.8 (H1oo)	Q8VIK3	Transcript			WB	[59, 60, 129]
H1.11	F7DCP6	Inferred
Macro-H2A.3	Q9D3V6	Transcript
H2A.L.1 gene: H2al1b	A0A087WP11	Inferred	Yes (1)
H2A.L.1 gene: H2al1j	A2BFR3	Inferred
H2A.L.1 gene: H2al1 k	J3QP08	Inferred
H2A.L.1 gene: H2al1 m	Q9DAD9	Transcript
H2A.L.1 gene: H2al1n	Q497L1	Transcript	Yes (2)
H2A.L.1 gene: H2al1o	L7MU04	Inferred
H2A.L.2 (H2A.B1,H2A.Lap3)	Q9CQ70	Transcript	Yes (3)	Yes	RT-PCR; NB; WB	[38, 39, 125]
Y-chr H2A.L.3	A9Z055	Transcript			RT-PCR	[98]
H2A.P (H2A.L3,H2A.Lap4,)	Q9CR04	Inferred			RT-PCR	[38, 39]
H2A.B.2	S4R1M3	Inferred			RT-PCR; WB	[40, 99]
H2A.B.3 gene: H2afb3	S4R1G7	Inferred			RT-PCR; WB	[40, 99]
H2A.B.3 (H2A.Lap1) gene: GM14920	S4R1E0	Inferred			RT-PCR; WB	[39, 40, 99]
H2B.L.2	Q9DAB5	Transcript	Yes (4)	Yes	RT-PCR; WB	[38]
H3.3 gene: GM6421	EDL18362.1	Predicted	Yes (1)	Yes	RT-PCR	[103]
H3.3 gene: GM10257	XP_003084990.1	Record removed	Yes (1)		RT-PCR	[103]
CENPA-cenH3	O35216	Transcript		Yes	qPCR; WB	[126, 127]
H3.5	P02301	Inferred			RT-PCR
TS H3.4 (H3t)	NP_001304932.1	Predicted		Yes	RT-PCR	[103]

The “protein status” was retrieved from UniProtKB: “Evidence at transcript level” (noted “Transcript”) or “Inferred from homology” (noted “Inferred”, update of July 2016). Three variants are predicted in NCBI database and are absent in UniProtKB. Information about the detection of some variants at the mRNA level (e.g. by RT-PCR) or at the protein level (e.g. by WB or MS) was further completed with publications and compared to the MS identification results obtained in the present study

NB northern blot, WB western blot, MS mass spectrometry