Table 2.
Candidate drought-responsive genes exhibiting differential expression in tobacco leaves in response to drought and re-watering treatments
| Gene ID | CL (RPKM) | DL (RPKM) | WL (RPKM) | log2Fold (DL/CL) | padj (DL/CL) | Regulation (DL/CL) | log2Fold (WL/DL) | padj (WL/DL) | Regulation (WL/DL) | Annotation |
|---|---|---|---|---|---|---|---|---|---|---|
| Response to stimulus and stress | ||||||||||
| gene_16580 | 0.7 | 46.91 | 0.37 | 5.81 | 8.59E-03 | Up | −6.74 | 2.11E-02 | Down | Late embryogenesis abundant protein |
| gene_64575 | 0.45 | 18.14 | 0 | 5.08 | 1.33E-02 | Up | -Inf | 6.63E-03 | Down | Adenine nucleotide alpha hydrolases-like superfamily protein |
| gene_58070 | 2.55 | 51.67 | 0.63 | 4.10 | 4.92E-02 | Up | −6.11 | 1.56E-02 | Down | MLP-like protein 423 |
| gene_71064 | 0 | 6.58 | 0.03 | Inf | 6.47E-05 | Up | −7.64 | 1.24E-02 | Down | heat shock protein 90.1 |
| gene_24848 | 0.01 | 7.64 | 0.03 | 8.86 | 9.01E-05 | Up | −7.87 | 8.14E-03 | Down | heat shock protein 90.1 |
| gene_29395 | 0.25 | 46.91 | 0.17 | 7.33 | 8.30E-04 | Up | −7.84 | 8.00E-03 | Down | heat shock protein 70B |
| gene_46118 | 0.32 | 278.97 | 0.55 | 9.52 | 8.52E-04 | Up | −8.72 | 2.19E-02 | Down | heat shock protein 21 |
| gene_81903 | 0.02 | 5.45 | 0 | 8.17 | 8.66E-04 | Up | -Inf | 1.29E-02 | Down | HSP20-like chaperones superfamily protein |
| gene_4627 | 0.91 | 49.75 | 0.98 | 5.53 | 4.38E-03 | Up | −5.41 | 4.14E-02 | Down | mitochondrion-localized small heat shock protein 23.6 |
| gene_57393 | 0.42 | 14.46 | 0.04 | 4.87 | 1.21E-02 | Up | −8.17 | 3.07E-03 | Down | heat shock protein 101 |
| gene_32 | 1.77 | 52.95 | 1.13 | 4.66 | 2.06E-02 | Up | −5.29 | 4.52E-02 | Down | mitochondrion-localized small heat shock protein 23.6 |
| gene_26545 | 0.7 | 18.74 | 0.33 | 4.50 | 2.10E-02 | Up | −5.56 | 2.82E-02 | Down | heat shock transcription factor A2 |
| gene_6132 | 0.59 | 15.69 | 0.32 | 4.50 | 2.19E-02 | Up | −5.37 | 3.54E-02 | Down | heat shock cognate protein 70-1 |
| Antioxidant metabolism | ||||||||||
| gene_31648 | 0 | 72.95 | 0 | Inf | 1.47E-05 | Up | -Inf | 9.23E-04 | Down | glutathione S-transferase TAU 19 |
| Cell Cycle | ||||||||||
| gene_7843 | 6.75 | 0.03 | 5.81 | −7.90 | 1.49E-04 | Down | 7.70 | 4.37E-03 | Up | minichromosome maintenance (MCM2/3/5) family protein |
| gene_72411 | 6.22 | 0.02 | 4.86 | −8.24 | 3.33E-04 | Down | 7.90 | 9.62E-03 | Up | Minichromosome maintenance (MCM2/3/5) family protein |
| gene_47817 | 6.08 | 0.05 | 4.62 | −7.25 | 6.94E-04 | Down | 6.87 | 1.73E-02 | Up | Minichromosome maintenance (MCM2/3/5) family protein |
| gene_39576 | 10.34 | 0.16 | 8.29 | −6.29 | 9.95E-04 | Down | 5.98 | 1.56E-02 | Up | minichromosome maintenance (MCM2/3/5) family protein |
| gene_3086 | 9.49 | 0.28 | 7.78 | −5.33 | 4.99E-03 | Down | 5.06 | 4.79E-02 | Up | Minichromosome maintenance (MCM2/3/5) family protein |
| gene_50222 | 4.61 | 0 | 5.25 | -Inf | 4.36E-03 | Down | Inf | 1.52E-02 | Up | Cyclin family protein |
| gene_69883 | 3.7 | 0 | 3.43 | -Inf | 4.42E-03 | Down | Inf | 2.72E-02 | Up | Cyclin B2 |
| gene_10225 | 5.91 | 0.13 | 7.51 | −5.70 | 1.01E-02 | Down | 6.06 | 2.63E-02 | Up | CYCLIN B2 |
| gene_64406 | 2.63 | 0 | 3.41 | -Inf | 2.12E-02 | Down | Inf | 3.34E-02 | Up | Cyclin family protein |
| gene_11897 | 3.75 | 0.07 | 5.52 | −6.05 | 2.41E-02 | Down | 6.63 | 2.82E-02 | Up | Cyclin family protein |
| Photosynthesis | ||||||||||
| gene_36062 | 58.44 | 0 | 91.53 | -Inf | 1.53E-05 | Down | Inf | 9.23E-04 | Up | chlorophyll binding (GO:0016168) |
| gene_36065 | 543.31 | 0.27 | 786.01 | −11.22 | 1.76E-02 | Down | 11.51 | >0.05 | Up | chlorophyll binding (GO:0016168) |
| gene_49443 | 130.35 | 0 | 292.05 | -Inf | 2.88E-05 | Down | Inf | 0.008004336 | Up | chlorophyll binding (GO:0016168) |
| gene_52444 | 252.59 | 0.32 | 477.85 | −9.88 | 1.53E-03 | Down | 10.54 | >0.05 | Up | chlorophyll binding (GO:0016168) |
| gene_62465 | 1.43 | 54.9 | 2.22 | 5.02 | 8.58E-03 | Up | −4.63 | >0.05 | Down | Chlorophyll A-B binding family protein |
| gene_62676 | 126.28 | 7.19 | 163.51 | −4.38 | 2.83E-02 | Down | 4.51 | >0.05 | Up | chlorophyll binding (GO:0016168) |
| gene_8497 | 5.7 | 0 | 6.12 | -Inf | 5.25E-03 | Down | Inf | 2.07E-02 | Up | chlorophyll binding (GO:0016168) |
| gene_8498 | 208.48 | 0.2 | 236.39 | −10.27 | 1.39E-03 | Down | 10.46 | 2.09E-02 | Up | chlorophyll binding (GO:0016168) |
| gene_8499 | 265.11 | 0.36 | 350.96 | −9.78 | 2.78E-03 | Down | 10.20 | 4.95E-02 | Up | chlorophyll binding (GO:0016168) |
| Protein phosphorylation | ||||||||||
| gene_43351 | 8.04 | 0.47 | 7.29 | −4.33 | 3.20E-02 | Down | 3.96 | >0.05 | Up | Protein kinase protein with adenine nucleotide alpha hydrolases-like domain |
| gene_50346 | 6.14 | 0.17 | 4.75 | −5.41 | 7.00E-03 | Down | 4.80 | >0.05 | Up | Concanavalin A-like lectin protein kinase family protein |
| gene_6225 | 0.65 | 24.87 | 0.21 | 5.02 | 1.50E-02 | Up | −6.64 | 1.29E-02 | Down | protein phosphorylation (GO:0006468) |
| gene_62579 | 12.18 | 0.12 | 17.21 | −6.85 | 5.07E-04 | Down | 7.37 | 4.45E-03 | Up | Protein kinase superfamily protein |
| gene_63746 | 16.12 | 0.59 | 20.63 | −5.02 | 8.73E-03 | Down | 5.39 | 3.64E-02 | Up | Protein kinase superfamily protein |
| gene_65325 | 4.58 | 0.12 | 2.71 | −5.51 | 4.49E-03 | Down | 4.50 | >0.05 | Up | Concanavalin A-like lectin protein kinase family protein |
| gene_73172 | 1 | 0.01 | 0.86 | −6.53 | 4.56E-02 | Down | 6.43 | >0.05 | Up | Protein kinase family protein with ARM repeat domain |
| gene_81940 | 5.89 | 0.05 | 6.12 | −7.22 | 7.22E-03 | Down | 7.28 | 3.29E-02 | Up | cyclin-dependent kinase B1 |
| gene_45400 | 0.16 | 3.36 | 0 | 4.39 | >0.05 | Up | -Inf | 1.89E-02 | Down | protein phosphorylation (GO:0006468) |
| gene_51323 | 1.76 | 0 | 3.51 | -Inf | >0.05 | Down | Inf | 4.91E-02 | Up | Serine_threonine-protein kinase 6 |