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. 2017 Jan 10;17:33. doi: 10.1186/s12906-016-1510-5

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Spatiotemporal activity maps of optical activity during recording time. The five images in each row indicate frames consisting of every 100^th frame in recording time. a Following electrical stimulation of the receptive field before EA in nerve-injured rats, significantly activated signals were detected. b Following electrical stimulation of the receptive field immediately after EA, intensity and activated area of fluorescence were typically reduced. c, d, and e Reduced and weak signals were observed 30, 60, and 90 min after EA. f and g By 120 and 150 min after EA, optical signals gradually recovered (C: caudal, A: anterior and L: lateral, scale bar = 800 μm)