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. 2017 Jan 10;17:33. doi: 10.1186/s12906-016-1510-5

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Changes of fluorescence intensity and activated area after EA stimulation. a Activated optical signals had different colors (red, yellow, and green). b Using the MetaMorph program, images were converted from colorful images to singular (red) color images (scale bar, 800 μm). Then activated area were calculated in the captured S1 cortex. c Each activated signal in pre- or post-EA stimulation was detected after electrical stimulation of the hind paw in nerve-injured rats. The average peak fluorescence is shown. After EA stimulation, the activated fluorescence signals became typically smaller and reduced. d Time-dependent changes in the maximal activated area in S1 cortex. Changes of fluorescence area at pre- and post-EA stimulations are presented (P < 0.05, n = 10)